Figure 6. SIRT6 inhibition prevents moDC differentiation. (A-C) Human adherent PBMCs were cultured with GM-CSF and IL-4 for 6 days in the presence of either S6 (at the indicated concentrations) or vehicle DMSO. Thereafter, cells were harvested, washed and analyzed by flow cytometry for CD14 and CD1a expression or utilized as stimulators in MLR. (A) One representative experiment out of five is presented. (B, C) Results are means ± SEM of those obtained with five different donors. *: p<0.05; ***: p<0.001.