Research Paper Volume 8, Issue 2 pp 231—244

Reversal of phenotypes of cellular senescence by pan-mTOR inhibition

Figure 1. AZD8055 causes major changes in near-senescent fibroblast morphology and size. HF043 fibroblasts at high CPD were incubated with AZD8055 or equivalent volume of DMSO for 7 days. (A) Cells were observed in situ by phase contrast microscopy. (B) Magnified images from (A). (C) Cells were harvested by trypsinization and cell suspension analysed using a Cellometer T4; images were obtained at x20 magnification.