Research Paper Volume 8, Issue 1 pp 192—207

miR-320a mediates doxorubicin-induced cardiotoxicity by targeting VEGF signal pathway


Figure 3. Inhibition of miR-320a improved doxorubicin induced endothelial cells impairment in vitro. (A) Proliferation detected by BrdU incorporation assays. (B) Apoptosis measured by Annexin V/PI flow cytometric analysis. (C) NO release detected by Nitric oxide colorimetric assays. (D) Tube formation determined on Matrigel. (E) Migration evaluated by transwell experiment. Scale bar, 200μm. Data are representative of three experiments. Data are expressed as mean ± SEM, n≥3, *P<0.05 versus control, #P<0.05 versus Doxo + negative con, $P<0.05 versus negative control.