Figure 8. Effect of age and reloading on autophagic flux and p62 protein accumulation using the autophagy inhibitor colchicine. Representative Western blots (A) and quantification of LC3B-II protein expression in the soleus (B) and tibialis anterior (TA) (C) muscles of control and 7 day reloaded adult (9 mo) and old (29 mo) rats treated with or without colchicine. Total protein, determined by stain-free imaging of the PVDF membrane, was used to normalize protein expression. Data are expressed as a percentage relative to the adult control group for each protein (n=3-6/group). Values are mean ± SEM, *p<0.05.