Research Paper Volume 8, Issue 1 pp 127—146

Age-related deficits in skeletal muscle recovery following disuse are associated with neuromuscular junction instability and ER stress, not impaired protein synthesis

Figure 8. Effect of age and reloading on autophagic flux and p62 protein accumulation using the autophagy inhibitor colchicine. Representative Western blots (A) and quantification of LC3B-II protein expression in the soleus (B) and tibialis anterior (TA) (C) muscles of control and 7 day reloaded adult (9 mo) and old (29 mo) rats treated with or without colchicine. Total protein, determined by stain-free imaging of the PVDF membrane, was used to normalize protein expression. Data are expressed as a percentage relative to the adult control group for each protein (n=3-6/group). Values are mean ± SEM, *p<0.05.