Figure 5. Rapamycin induces differential temporal regulation on cardiac metabolome. Venn diagram (A) and heatmap (B) of metabolite changes for different durations of rapamycin treatment. Rapamycin differentially regulated levels of metabolites in glycolysis (C), BCAA (D), glutamine anaplerosis (E) and aspartate anaplerosis (F). n≥5/group. * p <0.05 vs. Ctrl. Data are represented as mean ± SEM.