Figure 4. p21 silencing 11 days after IR with sample collection 2 days later (13d). (A) relative p21 levels. (B) relative EdU incorporation. (C) relative Cdk2 levels. All values are scaled to day 0. Light and dark gray bars indicate 10 and 20 Gy radiation, respectively. Error bars indicate standard error of the mean (SEM) (n≥3). (D) representative western blot demonstrating validation of the p21 silencing in irradiated MRC5 cells (20 Gy).