Research Paper Volume 8, Issue 1 pp 178—191

Ghrelin receptor regulates adipose tissue inflammation in aging

Figure 1. GHS-R ablation shifts peritoneal macrophages of old mice toward anti-inflammatory state, and releases more norepinephrine. Young (4-5 months) and old (13-16 months) mice were used. (A) Expression of Ghsr gene in different tissues from WT mice. Hypo: hypothalamus; PM: peritoneal macrophages; BM: bone marrow; Pan: pancreas; Mus: skeletal muscle; BAT: brown adipose tissue; WAT: white adipose tissue. (B) Expression of Ghsr and F4/80 genes in PM of young and old WT mice. (C) Expression of macrophage-related genes in PM of old WT and Ghsr−/− mice. (D) M1-like and M2-like macrophages, as well as ratio of M1-like/M2-like macrophages in PM of old WT and Ghsr−/− mice. (E) Norepinephrine (NE) levels in PM of old WT and Ghsr−/− mice. N = 6-10. &, p<0.05, &&, p<0.001, other tissue vs. hypothalamus; #p<0.05, ##p<0.001, old vs. young WT; *p<0.05, **p<0.001, WT vs. Ghsr−/−.