Figure 3. GHS-R ablation reduces macrophage infiltration into WAT, decreases pro-inflammatory M1-like macrophages and reduces M1/M2 macrophage ratio in WAT of old mice. Young (4-5 months) and old (13-16 months) mice were used. Total macrophages (A), M1-like macrophages (B), M2-like macrophages (C), and the ratio of M1-like/M2-like macrophages (D) in epididymal WAT from old WT and Ghsr−/− mice. (E) Immunohistochemical staining of epididymal WAT from old WT and Ghsr−/− mice with anti-F4/80 antibody. Red arrows point to the “crown-like structures” macrophages. (F) Ex vivo lipolysis of WAT of old WT and Ghsr−/− mice, treated with or without 10 μM isoproterenol. N = 6-10. ##p<0.001, old vs. young WT; *p<0.05, **p<0.001, WT vs. Ghsr−/−.