Figure 4. GHS-R ablation decreases age-associated increases of inflammation in BAT. Young (4-5 months) and old (13-16 months) mice were used. Expression of F4/80 (A), MCP1 (B) and TNF-α (C) genes in BAT from young and old WT and Ghsr−/− mice. Expression of IL-1β (D), CD11c (E) and CD206 (F) genes in BAT of old WT and Ghsr−/− mice. N = 6-8. #p<0.05, ##p<0.001, old vs. young WT; *p<0.05, **p<0.001, WT vs. Ghsr−/−.