Research Paper Volume 8, Issue 1 pp 178—191

Ghrelin receptor regulates adipose tissue inflammation in aging

Figure 5. GHS-R affects inflammatory responses in macrophages in vitro. (A) GHS-R antagonist suppressed TNF-α and IL-1β gene expression in LPS (1 μg/ml)-treated RAW264.7 cells with or without GHS-R antagonist D-Lys3 GHRP-6 (10 μM). N = 6. *p<0.05, **p<0.001, LPS + Lys3 GHRP-6 vs. LPS control. (B) Expression of Ghsr in shGHS-R knockdown RAW264.7 cells. (C) Expression of F4/80, MCP1, TNF-α, IL-1β, CD11c and CD206 in shGHS-R knockdown RAW264.7 cells. shScramble represents scramble shRNA; shGHS-R represents GHS-R specific shRNA. N = 9. *p<0.05, **p<0.001, shScramble vs. shGHS-R.