Research Paper Volume 8, Issue 4 pp 642—661

The effects of graded levels of calorie restriction: VI. Impact of short-term graded calorie restriction on transcriptomic responses of the hypothalamic hunger and circadian signaling pathways


Figure 5. Effect of graded calorie restriction on circulating hormone levels and genes involved in circadian rhythm. (A) Genes involved in circadian rhythm pathways based on their log fold change relative to ad libitum feeding for 12h per day (12AL) and their correlation coefficient with circulating hormone levels. Blue indicates down-regulation and red upregulation relative to 12AL. Purple indicates a negative correlation coefficient and green a positive correlation with circulating hormone levels. 10CR, 20CR, 30CR and 40CR refers to 10 %, 20 %, 30 % and 40 % restriction and 24AL refers to 24h ad libitum feeding. (B) Expression levels of core clock genes plotted against circulating hormone levels. Those genes with a significant correlation based on p-value < 0.05 are included in the plot. (C) Overview of core clock genes correlating with circulating hormone levels. The lines connecting hormones and genes represent correlations and the width of these lines indicate the strength of the correlation coefficient. Green indicates a positive correlation and purple a negative correlation.