Figure 5. Contribution of DDR for MICA and ULBP2 expression. (A,B) Growing cells were treated with either the ATM inhibitor KU60019 (10μM, A) or the ATM/ATR inhibitor Caffeine (5mM, B) for 1 hour prior to Etoposide treatment (100μM). Twenty four hours later the level of MICA and ULBP2 expression were assessed by RT-PCR. (C,D) DIS IMR-90 cells were treated with KU60019 (10μM) for 24, 48, 72 and 96 hours and the expression of MICA (C) and ULBP2 (D) was assessed at each time point via RT-PCR. Data presented as mean with S.E.M of three independent experiments. Two-tailed t-test *P<0.05, **P<0.001, ***P<0.0001.