Research Paper Volume 8, Issue 2 pp 328—344

NKG2D ligands mediate immunosurveillance of senescent cells

Figure 5. Contribution of DDR for MICA and ULBP2 expression. (A,B) Growing cells were treated with either the ATM inhibitor KU60019 (10μM, A) or the ATM/ATR inhibitor Caffeine (5mM, B) for 1 hour prior to Etoposide treatment (100μM). Twenty four hours later the level of MICA and ULBP2 expression were assessed by RT-PCR. (C,D) DIS IMR-90 cells were treated with KU60019 (10μM) for 24, 48, 72 and 96 hours and the expression of MICA (C) and ULBP2 (D) was assessed at each time point via RT-PCR. Data presented as mean with S.E.M of three independent experiments. Two-tailed t-test *P<0.05, **P<0.001, ***P<0.0001.