Research Paper Volume 8, Issue 3 pp 484—505

MicroRNA-15b regulates mitochondrial ROS production and the senescence-associated secretory phenotype through sirtuin 4/SIRT4


Figure 11. The miR-15b - SIRT4 axis regulates the expression of components of the senescence-associated secretory phenotype (SASP). Inhibitors against miR-15b were transfected in primary human dermal fibroblasts in the absence or presence of siRNA duplexes against SIRT4. Transcript levels of Interleukin-6 (IL-6) (A), Interleukin-8 (IL-8) (B), Interleukin-1α (IL1α) (C), Interleukin-1β (IL1β) (D), Interferon γ (IFNγ) (E), vascular endothelial growth factor (VEGF) (F), and matrix metalloproteinase-1 (MMP1) (G) were analysed by qRT-PCR. Shown are mean ± s.d. values from nine to thirteen experiments (except C, D, and E, where 5-6 independent experiments were performed). To evaluate statistical significance, ANOVA on ranks Dunn´s tests were performed (*p<0.05).