Research Paper Volume 8, Issue 4 pp 697—708
Sex-specific effects of cytotoxic chemotherapy agents cyclophosphamide and mitomycin C on gene expression, oxidative DNA damage, and epigenetic alterations in the prefrontal cortex and hippocampus – an aging connection
Figure 4. Altered DNA methylation in the PFC and hippocampus tissues of chemotherapy-exposed animals. (A) Levels of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in the genomic DNA of the in the PFC and hippocampus tissues of chemotherapy-exposed animals. Mean ± SD, n = 4; *p<0.05, Student's t-test. (B) Levels of DNMT1, DNMT3a and MeCP2 in the PFC tissues of chemotherapy-exposed female animals 3 weeks after treatment. Lysates from PFC tissues were immunoblotted using antibodies against DNMT1, DNMT3a, MeCP2 and actin. *p<0.05, Student's t-test.