Research Paper Volume 8, Issue 4 pp 777—795

Aging causes decreased resistance to multiple stresses and a failure to activate specific stress response pathways


Figure 6. Activation of heat shock response (HSR), mitochondrial unfolded protein response (mitoUPR) and endoplasmic reticulum unfolded protein response (ER-UPR) lasts for multiple days after induction. Fluorescent reporters strains were used to monitor the activation of the HSR, mitoUPR and ER-UPR following exposure to stress on day 1 of adulthood. Fluorescent reporter activity was monitored daily. (A) The HSR, as measured by the Phsp-16.2::GFP reporter strain, remains activated for at least 5 days following a mild 35°C heat stress for 2 hours. (B) The mitoUPR, as measured by the Phsp-6::GFP reporter strain, remains active for at least 7 days following exposure to 4 mM paraquat for 1 day (oxidative stress). (C) The ER-UPR remains active for only 2 days after exposure to 5 μg/ml tunicamycin for 1 day (ER stress). Error bars indicate SEM. *p<0.05, **p<0.01, ***p<0.001.