Research Paper Volume 8, Issue 5 pp 945—957
Study of mitochondrial respiratory defects on reprogramming to human induced pluripotent stem cells
Figure 4. Characterization of the derived hiPSCs. (A) Cell morphology and immunocytochemistry analysis of pluripotent markers OCT4 and TRA-1-60 in a representative control hiPSC line (MRU11780) and LHON hiPSC line (LHON Q1-4). Scale bar = 100μm. (B) In vitro embryoid body differentiation of hiPSC into cell representative of the endoderm (AFP), mesoderm (SMA) and ectoderm (NESTIN). Scale bar = 100μm. (C) hiPSCs form teratoma containing endodermal cells (e, gut-like epithelium), mesodermal cells (c, cartilaginous structure; a, adipose tissue) and ectodermal cells (nr, neural rosette). Scale bar = 50μm.