**Figure 4.** **The effects of age and stroke on peripheral lymphocytes 72 hours after 90 minute MCAO.** (**A**) There was an effect of stroke (F(1,46)=p<0.0001) on the ratio of circulating lymphocytes to myeloid cells, a measure of lymphopenia, as analyzed by 2-way ANOVA (n=10-15/group). This ratio was significantly reduced in young (p<0.05) and aged (p<0.0001) mice following stroke. (**B**) There was a significant effect of stroke (F(1,14)=p<0.005, n=3-6/group) but not age on the proportion of circulating CD69^{+} CD4^{+} T cells as analyzed by 2-way ANOVA. (**C**) The proportion of circulating CD8^{+} T cells expressing CD69 was affected by both stroke (F(1,14)=p=0.05) and age (F(1,14)=p<0.05) via 2-way ANOVA (n=3-6/group). Values are expressed as mean ± SEM. *, p≤0.05; ***, p<0.001.