Research Paper Volume 8, Issue 8 pp 1608—1624

A miR-335/COX-2/PTEN axis regulates the secretory phenotype of senescent cancer-associated fibroblasts


Figure 1. Genotoxic stress induces a pro-inflammatory SASP in normal human oral fibroblasts. Senescence-associated β-galactosidase activity (A) was measured in human oral fibroblasts treated with hydrogen peroxide (H2O2) and cisplatin at day 15 post-treatment and in replicative senescent and untreated presenescent control cells (n=4). qRT-PCR showed senescent fibroblasts expressed more cyclin dependent kinase inhibitor (CDKI): p16INK4A (B) and p21CIP1 (C) than presenescent controls (n=4). The heat-map demonstrates the cytokine expression profile of senescent fibroblasts measured by human cytokine antibody array (D) (n=2). All experiments were performed independently as indicated by n and with technical repeats. The bars represent mean ± STDEV (B, C) or mean ± SEM. *p<0.05, was determined by one-way ANOVA with post-hoc correction by Holm-Sidak method (A) and Dunn's method (B,C). (See Supplementary Figure S1).