Research Paper Volume 8, Issue 8 pp 1608—1624

A miR-335/COX-2/PTEN axis regulates the secretory phenotype of senescent cancer-associated fibroblasts


Figure 2. The SASP engenders a protumourigenic phenotype to the senescent oral fibroblasts. Soluble factors secreted by senescent fibroblasts stimulated proliferation and migration of D20 (A-B) and H357 cell lines (C-D) in 2D assay (n=3). The invasiveness of H357 cells was also increased by senescent fibroblasts in both 2D and 3D organotypic models (E-F) (n=3). Immunohistochemistry for p16INK4A was performed in paraffin embedded sections of 3D organotypic models to confirm presence of senescent fibroblasts (F, left lower panel). All experiments were performed independently as indicated by n and with technical repeats. The bars represent mean ± SEM. *p<0.05, was determined by one-way ANOVA with post-hoc correction by Tukey test (B) and Dunn's method (D-E). Two-way repeated measure ANOVA was performed to determine statistical significance in proliferation assay (A, C) with post-hoc correction by Holm-Sidak method. (See Supplementary Figure S2).