Research Paper Volume 8, Issue 8 pp 1690—1702

Loss of oxidative defense and potential blockade of satellite cell maturation in the skeletal muscle of patients with cancer but not in the healthy elderly


Figure 1. Fold change (±SD) in mRNA expression of genes related to (a) satellite cell markers (Pax3 and Pax7), (b) myoblast proliferation/early differentiation (MyoD and Myf5) and (c) late myocyte/myotube differentiation (MyoG) in the quadriceps muscle of C, HE and HMA groups: HMA (n=22), HE (n=17), CWS (n=18), CWL (n=10). Fold-change is relative to HMA participants. Statistical analysis was carried out on deltaCT values. Q-PCR results show upregulation of mRNA levels of Pax3 (p<0.0001 in HE, CWS and CWL) compared with HMA and Pax7 (p<0.0001 in HE; p<0.05 in CWS) compared with HMA. Expression of early myogenesis markers was increased: MyoD (p<0.0001 in HE and p<0.001 in CWS) and Myf5 (p<0.0001 in HE and CWS, P<0.005 in CWL) compared with HMA. The expression of MyoG is upregulated in HE (p<0.0001) compared with HMA and reduced in CWS and CWL (p<0.0001) compared with HMA.