Research Paper Volume 8, Issue 8 pp 1759—1780

Figure 1. SA extends the lifespan of N2 C. elegans worms. (A) Kaplan-Meier survival curves for concentration dependency of SA-mediated N2 lifespan extension. Upon day 1 of adulthood, SA was administered every 2 days and survival was assessed every other day until all the worms died. (B) Summary of SA treated N2 median lifespans. SA administration shows a dose-dependent increase in median lifespan. Data are expressed as means ± SEM from four independent experiments. *P < 0.05 as compared with vehicle control, **P < 0.01 as compared to vehicle control. (C) Effects of SA-mediated decreases in lipofuscin autofluorescence accumulation with age. SA response profiles were generated from integrating the area-under-the-curve (AUC) of fluorescent intensity as a function of time. Compared with N2 vehicle control, treatment with SA shows a significant reduction in autofluorescence. Data are expressed as means ± SEM from five independent experiments. *P < 0.01 as compared with vehicle control, **P < 0.005 as compared to vehicle control. (D) Changes in pharyngeal pumping rate of aging worms. Pumping rate declines with age, however SA administration retards decline in pumping rate. Data are expressed as means ± SEM from five independent experiments. *P < 0.05 as compared with vehicle control, **P < 0.01 as compared to vehicle control.