Research Paper Volume 9, Issue 2 pp 340—352

Protective effects of alpha phenyl-tert-butyl nitrone and ascorbic acid in human adipose derived mesenchymal stem cells from differently aged donors

Figure 2. DNA damage in ADSCs treated with H2O2 or UV light. (A) Tail length was quantified in ADSCs derived from young (20-40 years), middle-aged (41-60 years), and older (61+ years) after treatment with H2O2 for 30 minutes. Tail length was measured at the 30 minute (initial DNA damage) and 4 hour (end DNA damage) timepoints. Although tail length was longer in the ADSCs derived from 61+ year old donors, the tail length in differently aged ADSCs was statistically comparable. (B) Tail length was measured in ADSCs from differently aged sources following treatment with UV light for 30 minutes. Tail length was statistically comparable in ADSCs derived from young, middle-aged, and older donors. In comparison to ADSCs treated with H2O2, tail length was drastically shorter in ADSCs treated with UV light.