Research Paper Volume 9, Issue 2 pp 340—352

Protective effects of alpha phenyl-tert-butyl nitrone and ascorbic acid in human adipose derived mesenchymal stem cells from differently aged donors

Figure 3. PBN and AA reduce DNA damage in H2O2-treated ADSCs. ADSCs from young, middle-aged, and older sources were preincubated for 24 hours with either PBN or AA and then treated with H2O2 for 30 minutes. (A) ADSCs preincubated with either PBN or AA displayed comparable % tail lengths to the controls, which were treated with H2O2 alone and not preincubated with PBN or AA. (B) ADSCs preincubated with either PBN or AA showed a significant reduction in % tail DNA compared to the controls. This was true for ADSCs derived from each age group. * indicates p < 0.05.