Research Paper Volume 9, Issue 2 pp 340—352

Protective effects of alpha phenyl-tert-butyl nitrone and ascorbic acid in human adipose derived mesenchymal stem cells from differently aged donors

Figure 4. OTM was low in the majority of H2O2-treated ADSCs preincubated with PBN or AA. Olive tail movement (OTM) at the 0 hour, 30 minute, 2 hour, and 4 hour timepoints was measured in H2O2-treated ADSCs additionally treated with PBN or AA. The % of the ADSC population showing an OTM < 6 (black), an OTM between 6 and 25 (grey), or an OTM > 25 (red) is shown at each timepoint. (A) In general, the OTM was quite low (< 6) in ADSCs treated with PBN. Higher OTMs were found at the 30 minute timepoint in ADSCs derived from 20-40 year old donors. (B) Similarly, the majority of ADSCs treated with AA showed an OTM < 6. Higher OTMs were observed at the 30 minute timepoint in ADSCs derived from 20-40 year old and 61+ year old donors.