Research Paper Volume 8, Issue 10 pp 2308—2323

Figure 3. SIRT6 decreases p27 protein levels. (A) Western blot analysis of SIRT6, p16, p21, p27, p53 and PTEN expression levels was carried out in SIRT6 overexpressing or knockdown 293 (left) and 2BS (right) cells. (B) Real-time PCR analysis of SIRT6, p27 was performed in cells as in A. Specific real-time PCR primers were used. (C) The protein half-life of p27 was evaluated in 293 cells with altered SIRT6 expression. (D) The protein half-life of p27 was evaluated in 2BS cells with SIRT6 overexpression.