Research Paper Volume 9, Issue 1 pp 26—40

Transcriptional regulation of PNPLA3 and its impact on susceptibility to nonalcoholic fatty liver Disease (NAFLD) in humans

Figure 3. Electrophoretic mobility shift assay (EMSA) for the rs139051 polymorphism. (A) Representative demonstration of EMSA assay for rs139051 sequence from three independent replications. Relative binding efficiency to nuclear extracts of the HepG2 cells between the A allele and G allele probes were shown (lane 1 and 3). The DNA-protein complex diminished in the reactions with 200X non-labeled competitor probes (lane 2 and 4). The interaction complex was specific to nuclear extract (lane 6) compared to the cytoplasmic extract (lane 5). The binding efficiency between nuclear proteins and the A allele was significantly higher compared to the G allele (p = 0.017). Data were shown in mean ± SD (B).