Research Paper Volume 8, Issue 12 pp 3185—3208

Figure 5d-f. Bcl-xL, decreases senescence, enhances proliferation and protects against oxidative damage in mouse embryo fibroblasts (MEFs) in primary culture. (d) Bcl-xLover-expression prevents cellular commitment to senescence by decreasing SA-β-Gal and expression of Dcr2. SA-β-Gal activity was measured by SA-β-Gal staining kit (n=3 in each group). RT-PCR expression analysis of Dcr2 in MEFs virally transduced with Bcl-xL orpMIG (controls) (n=7 each). (e) MEFs transduced with Bcl-xL show lower levels of lipid peroxidation, determined as malondialdehyde, MDA by HPLC (pMIGcontrols, n=6; Bcl-xL, n=11) (f) MEFs transduced with Bcl-xL show lower levels of oxidized proteins as measured by western blotting (pMIGcontrols, n=6; Bcl-xL, n=11). Data are expressed as mean ± SD. *P<0.05; **P<0.01; ***P<0.001.