Research Paper Volume 8, Issue 10 pp 2525—2537

Figure 1. Diminished expression of autophagy gene products in aged SVFs. (A) Relative mRNA expression of autophagy genes Beclin1, Atg3, Atg5, Atg7, Lc3a and Lc3b in adipose tissue SVF of young (4 m) and old mice (20 m) analyzed by real-time PCR using Ct values. Data represented in bar diagrams are Mean + SD value of relative mRNA expression from three independent experiments where total RNA was extracted from SVFs of young (n=5) and old (n=3) mice and used as a template for one-step RT-qPCR reaction. The significance levels *p<0.05, **p<0.001 or ***p<0.0001 were analyzed by unpaired student’s t-test using means and SDs. (B) Protein expressions of BECLIN1, ATG3 and ATG7 were analyzed by Western blotting of SVF lysates from young (n=5) and old (n=3) mice. Data presented here are representative image of three independent experiments. The relative density of protein bands were plotted in (C). Values were expressed as Mean + SD. of three independent experiments and the significance levels * p<0.05 or **p<0.001 were analyzed by Student’s t-test.