Research Paper Volume 8, Issue 10 pp 2551—2567

Figure 3. TERT mRNA and protein decrease during brain aging in mice while DR results in TERT accumulation in mitochondria. (A) TERT mRNA abundance in whole brain tissue from young (4 months) and old (21 months of age) mice (n=3-4 per group), t- test, *P<0.05. (B) TERT protein abundance in mouse brain tissue measured separately in cortex and cerebellum in young (6 months) and old (17 months) mice, (n=3 per group), t-test, *P<0.05, (C-E) TERT protein abundance in whole brain homogenates and in brain mitochondria in 3 independent DR experiments (as percentage of ad libitum fed controls, for details, see Table 1). Exp. 1: n=3 mice per group, exp. 2: n=4 mice per group, exp. 3: n=7 mice per group. t-test, *P<0.05.