Research Paper Volume 8, Issue 12 pp 3255—3271

Figure 3. SLM35 is involved in the regulation of the antioxidant system. (A) Indicated strains harboring a plasmid encoding SOD2-2HA under the control of its endogenous promoter were grown as before using SDC-URA. 50 µg of whole cell protein extracts were analyzed by Western blot using specific antibodies to detect Sod2 (HA) or G6PDH as loading control. Signals from three independent experiments were quantified by densitometry and are shown in Supplementary Figure S2. (B) Determination of catalase activity in log and stationary phase of wild-type, ∆slm35, ∆tor1 and ∆tor1∆slm35 strains. Cells were grown on SDC medium for 14-16 h (Log phase, left panel) or 3 days (Stationary phase, right panel). 50 µg of whole cell extracts were analyzed by native gel electrophoresis and catalase activity was determined as described in Materials and Methods. Cta1, catalase A; Ctt1, catalase T. Catalase from bovine liver from (HMW Native Marker Kit, GE Healthcare) was used as molecular weight marker and catalase activity control (first lane, left panel). A representative experiment out of three is shown.