Research Paper Volume 9, Issue 1 pp 68—97

Effects of an unusual poison identify a lifespan role for Topoisomerase 2 in Saccharomyces cerevisiae

Figure 11. Reduced Top2 activity extends replicative lifespan. (A) DeaD cells expressing top2-28 displayed a roughly two-fold increase in lifespan both in the absence or presence of LS1 compared to cells expressing native TOP2. (B) Anti-Top2 immunoblot of cell extracts from TOP2 and top2-28 expressing DeaD strains, using anti-G6PDH as the loading control. (C) Anti-Top2 immunoblot of cell extracts from Top2-DAmP and TOP2 strains using anti-G6PDH as the loading control. (D) Microdissection RLS assays of isogenic haploid strains expressing either Top2-DAmP or TOP2, obtained by sporulation the heterodiploid. (E) Budding indexes of terminal mother cells taken from the microdissection RLS assays of Top2-DamP and TOP2 strains shown in panel C.