Figure 2. LS1 shortens replicative lifespan by standard microdissection assay. Microdissection in the presence and absence of 1µM LS1 was performed as described in Materials and Methods. LS1 treated (●) FY839 cells had an average lifespan of 16.1 generations, a 41.5% drop from the untreated sample (○). Graph shows combined data from two independent FY839 colonies. Superimposed over the experimental data are modeled data showing the effect on the lifespan of the untreated sample of adding 1% (∆), 2% (○) and 3% (□) non-age-related cell death/generation.