Research Paper Volume 9, Issue 1 pp 68—97

Effects of an unusual poison identify a lifespan role for Topoisomerase 2 in Saccharomyces cerevisiae

Figure 2. LS1 shortens replicative lifespan by standard microdissection assay. Microdissection in the presence and absence of 1µM LS1 was performed as described in Materials and Methods. LS1 treated (●) FY839 cells had an average lifespan of 16.1 generations, a 41.5% drop from the untreated sample (○). Graph shows combined data from two independent FY839 colonies. Superimposed over the experimental data are modeled data showing the effect on the lifespan of the untreated sample of adding 1% (∆), 2% (○) and 3% (□) non-age-related cell death/generation.