Figure 3. Lifespan shortening activity of LS1 is independent of 2μ circles and Sir2. DEAD assays were performed in the presence and absence of 5µM LS1 (A) or 1mM NAM (B) in parental (BB579), fob1∆, or sir2∆ strains as described in Materials and Methods. Data represent the average of three biological replicates. Error bars represent standard deviation.