Research Paper Volume 9, Issue 1 pp 68—97

Effects of an unusual poison identify a lifespan role for Topoisomerase 2 in Saccharomyces cerevisiae

Figure 7. LS1 induces double strand breaks. (A) A pUC18 plasmid linearization assay was used to determine if LS1 is capable of promoting formation of Top2cc containing double strand breaks. Assays were performed as described in Materials and Methods. (B) Percent linearized DNA quantified with GelQuant.NET 1.8.2 software.