Research Paper Volume 9, Issue 1 pp 98—113

Aging enhances liver fibrotic response in mice through hampering extracellular matrix remodeling

Figure 4. Pro-resolutive macrophages infiltrate young livers. (A) Kupffer cells were identified by F4/80 immunohistochemistry staining in young and old mice 48 and 96 hours after the last CCl4 injection (magnification 80x) (n=6/group). Scale bare 100µm. Hepatic gene expression of F4/80 (B) and, CD11b, Ccl2, Mif and Vegf (C) (Mean ± SEM) (n=6/group). (D) Hepatic genes expression of Mmp13, Cxcl9, Cx3cr1, Il12beta, Il10 and Tgf beta (n=6/group). Induction compared to untreated liver appears in red and repression in green. Statistical analysis was performed by two-way ANOVA for repeated measures (boxes) followed by Bonferroni’s post-hoc correction. *P<0.05; ***P<0.001 for differences between age groups.