Figure 1. Characterization of phenotypic and evaluation of S1PR1/STAT3 axis in middle-aged mice. Body weight (a) (n=8 per group) and epididymal fat (b) (n=7 per group). UCP1 mRNA levels (c) and coloration of BAT (d) (n=7–10 per group). The Clams equipment was used to determinate VO2(e), VCO2(f), RER (g), and ambulatory activity point to point during 12 hours in light and dark period (h) and mean of cycle light and dark (i) (n=8 per group). Western blots showing S1PR1 protein levels and STAT3 tyrosine phosphorylation (j) in the hypothalamus of young and middle-aged mice. The mice were fasted prior to hypothalamus extraction for 10 hours (n= 6 per group). The Student’s t-test was performed to evaluate data. ± SEM are shown in (a and f) ***p<.0001; (c) **p<0,0021; (b,e and g) *p<0.05. For ambulatory activity point to point, as determined by Student´s t-test where (h) *p<0.05.