Figure 3. Evaluation of S1PR1/STAT3 axis in the hypothalamus of middle-aged rats. Body weight (a) (n=6–8 per group) and epididymal fat pad weight (b) (n=5 per group). Image of BAT (n=6 per group) (c). Representative Western blots show: UCP1 expression (d) S1PR1 protein expression and STAT3 tyrosine phosphorylation in hypothalamus (e) in young and middle-aged rats. Rats were fasted for 10 hours prior to extraction of the hypothalamus for Western blots analysis (n=6 per group). The Student’s t-test was performed to evaluate data. means ± SEM are shown in (a) ***p<0.0001 to (b) ***p<0.0005.