Figure 6. The effects of SM on the protein and gene expression of estrogen receptor ERα and ERβ in the uterus and vagina of mice. Western-blot (A, C) and Realtime PCR (B, D) analysis was carried out as described in the Methods. Representative blots are shown above, and quantitative analyses are shown below. P values are for one-way analysis of variance (ANOVA) comparing treatment groups with untreated mice. (A, B) ***P < 0.001, **P < 0.01 and *P < 0.05 compared with the Con group; ###P < 0.001 and ##P < 0.01compared with the SM group or E2 group; ▴▴P < 0.01 compared with the ERα. (C, D) ***P < 0.001 compared with the Sham group; ###P < 0.001, ##P < 0.01and #P < 0.05 compared with the OVX group;∆∆∆P < 0.001 and ∆∆P < 0.01compared with the SM group or E2 group.