Research Paper Volume 9, Issue 2 pp 353—369

Hypothalamic ΔFosB prevents age-related metabolic decline and functions via SNS

Figure 1. Site specific overexpression of ΔFosB in the VHT. Mice received stereotaxic injections of either AAV-ΔFosB or AAV-GFP. Only right hemisphere was injected with viruses, left hemisphere was injected with saline (sham) and sacrificed 1 week post-surgically (n=3). Brain frozen sections were subjected to histological analysis (A) Immunofluorescent staining with anti-ΔFosB antibody followed by Alexa-fluor568 conjugated secondary antibody (red). Green fluorescence originates from ires-GFP-AAV backbone. X100 magnification. (B) Western blotting of gross brain sections with anti-ΔFosB using β-actin as housekeeping control. Blots of two mice per virus were shown.