Research Paper Volume 9, Issue 2 pp 353—369

Hypothalamic ΔFosB prevents age-related metabolic decline and functions via SNS

Figure 2. VHT overexpression of ΔFosB improves glucose profile despite lower insulin response. Mice were stereotaxically injected into VHT with AAV-ΔFosB or AAV-GFP and glucose metabolism assessed 8 weeks post-surgically (n=8). (A) Fasted and fed glucose levels (B) Fasted and fed insulin levels (C) GTT glucose (D) GTT insulin (E) Immunostaining of pancreas with anti-insulin antibody. Scale bar; 200μm. (F) Histomorphometry of insulin-stained pancreatic islets (G). qPCR analysis of isolated pancreatic islets. (H) GSIS test of isolated pancreatic islets from AAV-GFP and AAV-ΔFosB mice. (I) GSIS test of MIN-6 cells transfected with ΔFosB. Data are presented as mean ± SEM. *p<0.05, **p<0.01.