Research Paper Volume 9, Issue 2 pp 353—369

Hypothalamic ΔFosB prevents age-related metabolic decline and functions via SNS

Figure 5. ∆FosB exerts its favorable effects on glucose metabolism via blockade of AP1. Mice were stereotaxically injected into VHT with AAV-DNJunD or AAV-GFP and glucose metabolism was assessed 8 weeks post-surgically (n=4). (A) 6X TRE-driven luciferase reporting AP1 transactivation following transfection with FosB and JunD isoforms in mHypoE42 cell line. (B) Fasted and fed glucose levels (C) Fasted and fed insulin levels (D) GTT glucose (E) GTT insulin (F) ITT glucose (G) HOMA-IR. (H) Immunostaining of pancreas with anti-insulin antibody. Scale bar; 200μm. Histomorphometry of insulin-stained pancreatic islets. Data are presented as mean ± SEM. *p<0.05, **p<0.01.