Research Paper Volume 9, Issue 1 pp 256—285

Melatonin-micronutrients Osteopenia Treatment Study (MOTS): a translational study assessing melatonin, strontium (citrate), vitamin D3 and vitamin K2 (MK7) on bone density, bone marker turnover and health related quality of life in postmenopausal osteopenic women following a one-year double-blind RCT and on osteoblast-osteoclast co-cultures

Figure 8D-G. Effect of MSDK on sOPG and sRANKL expression in osteoblasts grown in transwell co-cultures, layered co-cultures and hMSCs monocultures. Treatments effects on secreted OPG (sOPG) and RANKL (sRANKL) were measured by ELISA in (D) transwells and (E) monocultures. Mean concentrations (pg/mL) of (i) sOPG: sRANKL, (ii) sOPG and (iii) sRANKL in culture media were analyzed, normalized against Os-/MSDK and compared between groups. Treatment effects on RANKL processing were detected via western blot by measuring the mean osteoblast’s expression of (i) 25 KDa and (ii) 24 KDa RANKL fragments in (F) transwells; and (G) 24 KDa RANKL fragment in layered co-cultures. *=p<.05, **=p<.01 and ***=p<.001; One-way ANOVA followed by Bonferroni’s post-hoc multiple comparison t-test (n=6 per group).