Research Paper Volume 9, Issue 2 pp 370—380

The expression of the BPIFB4 and CXCR4 associates with sustained health in long-living individuals from Cilento-Italy

Figure 3A-B. Migratory ability of LLI MNCs is impaired in non-healthy donors and associates with percentage of membrane CXCR4-positive cells. (A) Dot plot showing results of in vitro migration assay performed on a separate group of healthy (N=7) and non-healthy (N=5) LLIs. Number of MNCs migrated in the lower chamber toward vehicle (migration medium containing 0.1% serum bovine albumin, BSA) or chemoattractant SDF-1α (CXCL12) is shown. (B) Percentage of CXCR4 (SDF-1α receptor)-positive MNCs was measured by flow cytometry in migrated (MIG) and not migrated (NOT MIG) harvested from the upper and lower chamber of the migration system respectively. Results of the 12 donors are shown in the dot plot.