Figure 4. UPLC-MS-based metabolic profile analysis of the long-lived glp-1(e2141) mutants and the daf-16(mu86);glp-1(e2141) double mutants. Metabolomics analysis from UPLC-MS data for (A) YA and (B) 10A wild-type N2 and glp-1 (e2141), and for (C) YA and (D) 10A wild-type N2 and daf-16(mu86);glp-1(e2141) double mutants. Heatmap plot showed that 25 most importantly different metabolites from the comparison of the glp-1 mutants and N2. More information was listed in the Tables S3 and S4 (supplemental information). The detailed description of heatmap is as discussed in Figure 1B.