Research Paper Volume 9, Issue 2 pp 475—486

Long intergenic non-protein coding RNA 00858 functions as a competing endogenous RNA for miR-422a to facilitate the cell growth in non-small cell lung cancer

Figure 2. LINC00858 promotes tumor NSCLC cell growth in vitro. (A) Relative LINC00858 expression after transfection with sh-LINC00858 or pcDNA3.1-CT-GFP-LIN00858. (B) Statistical analysis of trypan blue staining. (C-D) CCK8 assays of A549 and SPC-A-1 cells after transfection. (E) Shown are representative photomicrographs of colony formation assay after transfection for fourteen days. Assays were performed in triplicate. *P < 0.05. Means ± SEM are shown. Statistical analysis was conducted using student t-test.