Figure 3. LINC00858 is a direct target of miR-422a. (A) Screen of the candidate miRNAs that target LINC00858 predicted by miRDB and PITA. (B) Sequence alignment of miR-422a with the putative binding sites within the wild-type regions of LINC00858. (C) Detection of miR-422a using qRT-PCR in the sample pulled down by biotinylated LINC00858probe. (D) Up-regulated miR-422a in A549 and SPC-A-1 cells, which stably over-expressed LINC00858, largely reversed the favorable effects of LINC00858 on cell proliferation. Assays were performed in triplicate. *P< 0.05. Means ± SEM are shown. Statistical analysis was conducted using student t-test.