Research Paper Volume 9, Issue 2 pp 315—339

Improved health-span and lifespan in mtDNA mutator mice treated with the mitochondrially targeted antioxidant SkQ1

Figure 1. Effect of SkQ1 treatment on appearance, kyphosis and alopecia in mtDNA mutator mice. (A) Pictures of mtDNA mutator mice: a non-treated (Mut) mouse and an SkQ1-treated (Mut+SkQ1) littermate female mouse, 248 and 256 days of age respectively. (B) Scores of kyphosis manifestation (arbitrary units) in mtDNA mutator mice. Each bar represents the mean ± S.E. score from mice of both genders: 21 Mut (10 females + 11 males) and 22 Mut+SkQ1 (13 f + 11 m) in ages 213-268 days; 11 Mut (5 f + 6 m) and 13 Mut+SkQ1 (6 f + 7 m) in ages 268–290 days; in ages 290–356 days, the number of mice decreased with age according to survival; the lowest number of mice in a group was 4 (2 f + 2 m). Day number under each bar indicates the center day of 11 days of mouse age. (C) X-ray picture of a non-treated mouse and an SkQ1-treated littermate female mtDNA mutator mouse (290 ± 4 days old). White circle arc indicates curvature angle measurements. Note that the angle is calculated from the extended line of the lower spine. (D) Angle of thoracic-lumbar curvature determined as in C. Each bar represents the mean ± S.E. from 10–11 mice of both genders in each group; Mut, 287 ± 7 days old, 5 f + 6 m; Mut + SkQ1, 284 ± 6 days old, 6 f + 5 m. (E) Bone minerality of spine cord area from scapula to lumbar. Each bar represents the mean ± S.E. from 6–8 female mice in each group. Mut, 290 ± 11 days; Mut + SkQ1, 287 ± 9 days. (F) Scores of alopecia manifestation in mtDNA mutator mice. The mice are the same as those in (B). * in B-F indicates a statistically significant difference between non-treated and SkQ1-treated mtDNA mutator mice (p < 0.05).