Research Paper Volume 9, Issue 2 pp 524—546

Compound effects of aging and experimental FSGS on glomerular epithelial cells

Figure 1. Albuminuria was higher in aged mice at baseline and in FSGS. (A-D) Sheep IgG staining. Sheep IgG staining confirmed the equal distribution of anti-glomerular antibody in the glomerular tuft in both young (Y) and aged (A) mice. Images were taken at 100x. (A'-D') 400x close-up images of glomeruli from (A-D). Sheep IgG deposition (purple color) was not seen in baseline mice as expected (A,B,A',B'). Animals given FSGS with the anti-glomerular antibody showed sheep IgG deposition in the glomerular tufts (C, D, C’, D'). (E) Albumin to creatinine ratios (ACR). ACRs (µg/mg) for young mice (black circles), and aged mice (white circles), at baseline prior to FSGS and at days 7, 14, 21 and 28 post-FSGS. ACR increased acutely for both young and aged animals to peak at D7, followed by gradual recovery for 21 days. Aged mice started with higher ACR at baseline and finished with significantly higher ACR at D28.