Research Paper Volume 9, Issue 3 pp 665—686

Lesion complexity drives age related cancer susceptibility in human mammary epithelial cells

Figure 6. Phospho-protein patterns as a function of age. Phospho-profiles of key proteins in the RTK, TNFα and TGFβ signaling pathway were assessed in all fifteen of the HMEC strains 9 days post-exposure to Cs and Ti ions. Roughly equitoxic doses of Cs and Ti were used for exposures (LD-CS = 0.12 Gy; HD-CS = 0.8 Gy; LD-Ti = 0.05 Gy; HD-Ti = 0.5 Gy). Four proteins that showed a change with age were plotted. These include pERK, pCREB, pAKT and total levels of TGFβRII. Dotted lines and solid lines represent regression lines. Trends exhibited with a low (A) and a high (B) dose of Cs are shown. Similarly, trends observed at a low (C) and a high (D) dose of Ti ions are shown. Data from two independent experiments were plotted for each radiation type and dose.