Research Paper Volume 9, Issue 3 pp 665—686

Lesion complexity drives age related cancer susceptibility in human mammary epithelial cells

Figure 7. Strain-specific differences in the frequency of centrosome aberrations. The fraction of ALDH+ cells were plotted relative to control for cells exposed to low and high doses of Cs (LD = 0.12 Gy; HD = 0.8 Gy) (A). Colored lines identify the symbol representing the same strain at low and high dose for two strains of comparable ages (16 y-green and 19 y-pink). Phospho-profiles of key proteins in the RTK, TNFα and TGFβ signaling pathway were compared between the two strains after Cs and Ti exposure (B). Fold change of high relative to low dose phospho-protein expression was plotted for the strains. Data represent 2 independent experiments. The fraction of cells with aberrant centrosomes (>3P) (C) and ALDH+ cells (D) was plotted relative to control for cells exposed to low and high doses of Cs and Ti ion. Roughly equitoxic doses of Cs and Ti were used for exposures (LD-CS = 0.12 Gy; HD-CS = 0.8 Gy; LD-Ti = 0.05 Gy; HD-Ti = 0.5 Gy). Trend lines show that the strains from the 66 y old and 68 y old respond uniquely to low and high doses. Phospho-profiles of key proteins in the RTK, TNFα and TGFβ signaling pathway were compared between the two strains post-exposure to Cs and Ti (E). Fold change comparing the high to low dose was plotted for both strains following each radiation exposure (magenta solid line= Ti ion 66 y old strain, magenta dotted line = Cs ion 66 y old, green solid line = Ti ion 68 y old, green dotted line = Cs 68 y old). Data represent two independent experiments for each radiation type.